Genetics Research

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Jeff A. Brady

Texas A&M AgriLife Research, 254-968-4144/Tarleton State University, 254-968-9221

jeffbrady@tamu.edu, brady@tarleton.edu

 

Professional Preparation

Ph.D.               Genetics          Texas A&M University, College Station Texas         2006

M.S.                Biology           Tarleton State University, Stephenville Texas           1998

B.A.                 English            Tarleton State University, Stephenville Texas           1993

 

Current Research Interests

  1. Microbial metagenomics/massively parallel DNA sequencing
  2. Using fire ant pathogens as biocontrol measures in conjunction with existing bait treatments
  3. Overlapping pathogens shared by native bees and honey bees
  4. Plant germplasm characterization/genotyping by sequencing
  5. Environmental impacts of concentrated animal feeding operations
  6. Antibiotic resistance genes in agricultural production systems

Previous Research Projects

  1. Map-based cloning of maturity gene loci in Sorghum bicolor
  2. Management tactics for Pierce’s disease of winegrape

 

Appointments

2012-present   Research Scientist, Texas A&M AgriLife Research, Stephenville, TX

2013-present   Assistant Professor, Department of Wildlife, Sustainability and Ecosystem Sciences, Tarleton State University, Stephenville, TX

 

Recent Publications and Patents

Painter, S.M., Pfau, R., Brady, J.A., and McFarland, A.M.S. 2013. Quantitative assessment of Naegleria fowleri and Escherichia coli concentrations within a Texas reservoir. Journal of Water and Health 11: 346-357. http://www.iwaponline.com/jwh/up/wh2013162.htm

Murphy, R.L., Morishige, D.T., Brady, J.A., Rooney, W.L., Yang, S., Klein, P.E., and Mullet, J.E. 2014. Ghd7 (Ma6) represses sorghum flowering in long days; Ghd7 alleles enhance biomass accumulation and grain production. The Plant Genome 7, 1-10. http://dx.doi.org/10.3835/plantgenome2013.11.0040

Harris-Schultz, K.R., Milla-Lewis, M., Zuleta, C., Schwartz, B.M., Hanna, W.W., and Brady, J.A. 2012. Development of simple sequence repeat markers and the analysis of genetic diversity and ploidy level in a centipedegrass collection. Crop Science 52: 1-10. https://www.agronomy.org/publications/cs/view/52-1/c11-03-0151.pdf

Noah, R.L., Muir, J.P, Brady, J.A., Wittie, R.D., Kattes, D.H., Pitman, W.D., Rea, G.L., and Brakie, M.R. 2012. Genotypic and phenotypic variability in three prairie acacia accessions. Crop Science 52: 951-959.

Foote, A.P., Lambert, B.D., Brady, J.A., and Muir, J.P. 2011. Phospate transporter expression in Holstein cows. Journal of Dairy Science 94, 1913-1916. http://www.ncbi.nlm.nih.gov/pubmed/21426981

Brady, J.A., Faske, J.B., Ator, R.A., Castañeda-Gill, J.M., and Mitchell, F.L. 2012. Probe-based real-time PCR method for multilocus melt typing of Xylella fastidiosa strains. Journal of Microbiological Methods 89, 12-17. http://dx.doi.org/10.1016/j.mimet.2012.02.002

Mullet, J.E., Rooney, W.L., Klein, P.E., Morishige, D., Murphy, R., and Brady, J. 2012. Discovery and utilization of sorghum genes (Ma5/Ma6). U.S. Patent 8309793 B2, November 13, 2012.

Harris-Shultz, K.R., Milla-Lewis, S.R., and Brady, J.A. 2012. Transferability of SSR and RGA markers developed in Cynodon spp. to Zoyzia spp. Plant Molecular Biology Reporter 30: 1264-1269.

Brady, J.A., Faske, J.B., Castañeda-Gill, J.M., King, J.L., and Mitchell, F.L. 2011. High-throughput DNA isolation method for detection of Xylella fastidiosa in plant and insect samples. Journal of Microbiological Methods. 86, 310-312. http://dx.doi.org/10.1016/j.mimet.2011.06.007

Murphy, R.L., Klein, R.R., Morishige, D.T., Brady, J.A., Rooney, W.L., Miller, F.R., Dugas, D.T., Klein, P.E., and Mullet, J.E. 2011. Coincident light and clock regulation of PRR37 controls photoperiodic flowering in sorghum. Proceedings of the National Academy of Sciences, USA 108, 16469-16474. http://www.pnas.org/content/108/39/16469.long

 

 

Students with current research projects in the lab:

Lauren Bassett
Graduate Student from Tarleton State University
Thesis: Survey detecting the variability of small subunit RNA of Kneallhazia solenopsae (Microsporidia: Thelohanidae) in the red imported fire ant, Solenopsis invicta, in South Texas
Future plans—Vet School

 

Jetton Fontenot

Jetton Fontenot
Graduate Student from Tarleton State University
Thesis: Determining the effects of terracing on soil health using microbial bioindicators
Now also working as a research scientist at the University of Texas, Austin
Future plans—Defend thesis and continue developing her career as a research scientist

 

Billy Fournier

Billy Fournier
Undergraduate student from Tarleton State University
Project: Python scripting for microbial bioinformatics pipeline/Computer Guru
Future plans—Programming job and possibly grad school

Louis Fowler
Graduate Student from Tarleton State University
Thesis: Identification of trypsin inhibitor genes in Sesbania vesicaria
Future plans—considering a Ph.D. at Texas A&M

 

Sheldon Hilaire

Sheldon Hilaire
Graduate Student from Tarleton State University
Thesis: Effects of antibiotics on the microbiome of forage bermudagrass following manure application
Future plans—Ph.D. program or enter the job market

 

Emily Lansmon

Emily Lansmon
Graduate Student from Tarleton State University
Thesis: Comparison of soil microbiomes of mesquite (Prosopis glandulosa)
and Texas wintergrass (Nassela leucotricha) communities
Future plans—Job/Family/More Grad School/maybe all 3

 

Morgan McDuffie

Morgan McDuffie
Graduate Student from Tarleton State University
Thesis: Characterization of expression of calpastatin isoforms in Callipyge sheep

Future plans—considering Ph.D. programs or a technical position

 

Cherilyn Porter
Graduate Student from Tarleton State University
Thesis: Survey detecting the presence of Nosema spp.  (Microsporidia: Nosematidae) in Texas Native bees and feral honey bees (Hymenoptera) found in Erath County, Texas
Future plans—Ph.D. program.
Justin Ralston
Undergraduate Student from Tarleton State University
Project: Microbial community structure of serpentinite derived soils
Future plans—probably a job in industry or the NRCS

 

Ben Seabolt
Graduate Student from Tarleton State University
Thesis: Differential gene expression of Streptococcus pneumoniae disease variants in vivo in a balb/c mouse model

Future plans—Job as a lab technician or maybe a Ph.D. program

 

Bianca Serrano

Bianca Serrano
Undergraduate Student Worker from Tarleton State University
Project: Tracking biological pollutants after dairy waste application
Future plans—Med School or Grad School

 

Sarah Shawver

Sarah Shawver
Graduate Student from Tarleton State University
Thesis: Proposal for variation of bacterial communities within a consociation soil map unit and a vegetative transect through an oak motte

Future plans—Ph.D. program

Former students and technicians:

1. Rebecca Ator
Undergraduate student from Tarleton State University
Project: DNA melt typing of Xylella fastidiosa strains
Now a GSS Technical Consultant at Informatica

2. Kristin Barnaby
Graduate Student from Tarleton State University
Thesis: Prevalence of Batrachochytrium dendrobatidis and the presence of inhibitory bacteria amongst Anuran species of Eastern Texas.
Now a Technical Manager at Alcon Laboratories

3. Tiana Blackmon
Graduate Student from Tarleton State University
Project: Effects of condensed tannins on grasshopper gut microbiota
Now working on her Ph.D.

4. Jessica Castaneda-Gill, almost Ph.D.
Graduate Student from Tarleton State University
Thesis: Multilocus melt typing of Xylella fastidiosa subspecies using real-time PCR
Now about to defend her dissertation at the University of North Texas Health Science Center, Ft. Worth

5. Brooke Cummings
Undergraduate Student Worker from Tarleton State University
Project: Microbial source tracking
Now an applicant to Veterinary School

6. Steffani Ellis
Undergraduate Student Worker from Tarleton State University
Project: Isolating/culturing fastidious microbes.
Now an Adjunct Professor at Tarleton State University

7. Nathan Elrod
Graduate Student from Tarleton State University
Thesis: The use of silver nanoparticles to develop a Tacaribe virus vaccine in mice
Now a graduate student in molecular biology at UTMB, Galveston

8. Jennifer Faske
Research Associate/Master bench scientist
Now a Laboratory Technician at Arkansas Children’s Hospital

9. Andrew Foote, Ph.D.
Graduate Student from Tarleton State University
Thesis: Characterization of NaPi-IIb phosphate transporter in the small intestine of dairy cattle and correlation to fecal phosphorus
Now an Animal Research Scientist with the USDA in Clay Center, Nebraska

10. Brent Fowler
Undergraduate Student Worker from Tarleton State University
Project: Detection of the Parasitic Microsporidian Kneallhazia solenopsae in Red Imported Fire Ant Colonies
Now a medical school student at Rocky Vista University, Parker, Colorado

11. Santiago Gallego
Stephenville High School student
Project: Bioinformatics technician
Now an undergraduate computer science major at Stanford University

12. Aaron Hassell
Graduate Student from the University of Nebraska, Lincoln
Project: Implementing a leaf disk assay for the study of Pierce’s disease transmission
Now a Research Associate with the Texas A&M AgriLife Research Center, Beeville Station

13. Jonathan King
Graduate Student from Tarleton State University
Thesis: Development and characterization of a Xylella fastidiosa strain collection
Now the Laboratory Manager at the Institute of Applied Genetics, University of North Texas Health Science Center, Ft. Worth

14. Rece Laney, M.D.
Undergraduate Student Worker from Tarleton State University
Project: Labeling Xylella fastidiosa strains with autofluorescent proteins
Now a general surgery resident at UTMB Health Science Center, Houston

15. Sarah Mays
Undergraduate Student from Tarleton State University
Project: The microbiome of spinose ear ticks collected from exotic wildlife in Somervell County, Texas
Now a Research Technician at the University of South Dakota

16. Arielle McEwin
Graduate Student from Tarleton State University
Thesis: Effects of condensed tannins on rumen microbiota of beef cattle
Now a High School Science Teacher

17. Jamie McGee-Woodard
Undergraduate student worker from Tarleton State University
Now a Lead Medical Technologist with Piedmont Healthcare

18. Sarah McKamie
Graduate Student from Tarleton State University
Project:
Now in Vet School at Texas A&M University

19. Matt Mears
Undergraduate Student Worker from Tarleton State University
Project: Microbial Source Tracking by Sequencing
Now a Laboratory Technician at Texas Women’s University

20. Blake Myers
Graduate Student from Tarleton State University
Thesis: Development of a high throughput assay for genotyping Xylella fastidiosa subspecies using multiplex PCR
Now a high school science teacher and all-around character

21. Ray Noah
Graduate Student from Tarleton State University
Thesis: Genotypic and Phenotypic Variability in Three Prairie Acacia Accessions
Now employed at StatOil

22. Stephanie Painter
Graduate Student from Tarleton State University
Thesis: Use of quantitative real-time pcr (qPCR) to investigate a potential correlation between populations of the pathogenic amoeba Naegleria fowleri and the bacterium Escherichia coli in Lake Granbury, Texas
Now a Senior Research Associate at The Texas Institute for Applied Environmental Research

23. Elias Sanchez
Stephenville High School student
Project: Bioinformatics technician
Now an undergraduate engineering major at Texas A&M University

24. Billie Shine
Undergraduate Student Worker from Tarleton State University
Project: Molecular identification of fire ant monogyne/polygyne colonies
Now a medical school student at Lake Erie College of Osteopathic Medicine

25. Skylar Shrum-Ballinger
Undergraduate Student Worker from Tarleton State University
Project: Jack-of-all-trades laboratory technician
Now in grad school at Sam Houston State University

26. Savannah Terrell
Undergraduate Student from Tarleton State University
Project: The microbiome of vertebrate scats in the Bosque River Watershed
Now a Medical Laboratory Technology student at Tarleton State University Ft. Worth Campus

27. Ashley Wallace
Undergraduate Student Worker from Tarleton State University
Project: Wolbachia detection in insect species
Now in Nashville, Tennessee

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